BacMet Experimentally Confirmed database

Result: Details of the source of prediction- mdfA/cmr

Gene_nameExperimentally Verified Resistance Gene Information Predicted Resistance Gene Information
mdfA/cmr
  • BacMet ID: BAC0208
  • Code for: Effux
  • Family: Major facilitator Superfamily¬†(MdfA family)
  • Sequence: FASTA
  • Cross-database IDs: Link
  • Organism: Only gram -ve, E. coli (Strain K12)
  • Location: Chromosome
  • Compound: Benzylkonium Chloride (BAC) [class: Quaternary Ammonium Compounds (QACs)], Ethidium Bromide [class: Phenanthridine], Acriflavine [class: Acridine], Tetraphenylphosphonium (TPP) [class: Quaternary Ammonium Compounds (QACs)], Sodium Hydroxide (NaOH) [class: Base], Rhodamine 6G [class: Xanthene]
  • Description: Multidrug transporter MdfA, Efflux pump driven by the proton motive force. Confers resistance to a broad spectrum of chemically unrelated drugs. Confers resistance to a diverse group of cationic or zwitterionic lipophilic compounds such as ethidium bromide, tetraphenylphosphonium, rhodamine, daunomycin, benzylkonium, rifampicin, tetracycline, puromycin, and to chemically unrelated, clinically important antibiotics such as chloramphenicol, erythromycin, and certain aminoglycosides and fluoroquinolones. OverExpression results in isopropyl-beta-D-thiogalactopyranoside (IPTG) exclusion and spectinomycin sensitivity. Transport of neutral substrates is electrogenic, whereas transport of cationic substrates is electroneutral. In addition to its role in multidrug resistance, confers extreme alkaline pH resistance, allowing the growth under conditions that are close to those used normally by alkaliphiles. This activity requires Na+ or K+; also known as Chloramphenicol resistance pump Cmr
  • Length (amino acid): 410
  • Reference: Edgar et al. 1997; Pubmed- 9079913, Nishino et al. 2001; Pubmed- 11566977, Sulavik et al 2001; Pubmed- 11257026

  • Similar resistance genes in 'BacMet Predicted database'


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