BacMet Database

BacMet Experimentally Confirmed database

Result: Details of the source of prediction- blt

Gene_name	Experimentally Verified Resistance Gene Information	Predicted Resistance Gene Information
blt	BacMet ID: BAC0050 Code for: Efflux Family: MFS Superfamily (TCR/tet family) Sequence: FASTA Cross-database IDs: Link Organism: Bacillus subtilis (strain 168) Location: Chromosome Compound: Ethidium Bromide [class: Phenanthridine], Rhodamine 6G [class: Xanthene], Acridine Orange [class: Acridine], Tetraphenylphosphonium (TPP) [class: Quaternary Ammonium Compounds (QACs)] Description: Energy-dependent efflux pump responsible for decreased drug accumulation in multi-drug-resistant cells. Probably uses a transmembrane proton gradient as the energy source. Causes the efflux of a variety of toxic substances, including such structurally diverse compounds as ethidium bromide, rhodamine and acridine dyes, tetraphenylphosphonium, puromycin, chloramphenicol, doxorubicin, and fluoroquinolone antibiotics. Length (amino acid): 400 Reference: Ahmed et al. 1995; Pubmed- 7608059	Similar resistance genes in 'BacMet Predicted database'
bltD	BacMet ID: BAC0051 Code for: Enzyme Family: Contains 1 N-acetyltransferase domain Sequence: FASTA Cross-database IDs: Link Organism: Bacillus subtilis (strain 168) Location: Chromosome Compound: Spermidine [class: Polyamines], Spermine [class: Polyamines] Description: Spermine/spermidine acetyltransferase bltD; Acetylates both spermidine and spermine at primary propyl amine moieties, with spermine being the preferred substrate. Length (amino acid): 152 Reference: Ahmed et al. 1995; Pubmed- 7608059	Similar resistance genes in 'BacMet Predicted database'
bltR	BacMet ID: BAC0052 Code for: Regulator Family: Contains 1 HTH merR-type DNA-binding domain Sequence: FASTA Cross-database IDs: Link Organism: Bacillus subtilis (strain 168) Location: Chromosome Compound: Ethidium Bromide [class: Phenanthridine], Rhodamine 6G [class: Xanthene], Acridine Orange [class: Acridine] Description: Multidrug-efflux transporter 2 regulator; Activates transcription of the blt gene in response to structurally dissimilar drugs. Length (amino acid): 273 Reference: Ahmed et al. 1995; Pubmed- 7608059	Similar resistance genes in 'BacMet Predicted database'

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